Generator

Part:BBa_K566029

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-27)

CcaR gene (constitutive expression)

CcaR is the response regulator of CcaS, wich is a member of cyanobacteriochrome family of proteins. Phycocyanobillin (PCB) binds at a conserved cysteine domain of CcaS and activates signaling activity. CcaS, along CcaR, activates transcription of promoter pCpcG2. Activation shows max response with 535 nm wavelength green light and with 672 nm wavelength red light. When absorbing green light, CcaS autophosphorylates and then carries out phosphotransfer to CcaR. Phosphorylated CcaR (CcaR*) acts as a transcription factor for pCpcG2. This process can be reverted with the absorption of red light.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 69
    Illegal SpeI site found at 765
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 69
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 765
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 69
    Illegal BamHI site found at 466
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 69
    Illegal SpeI site found at 765
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 69
    Illegal SpeI site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None